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Key Advantages
• High-throughput
• Safe & Stable
• Time saving
Product Description
Many studies have proved that SARS-CoV-2 enters target cells by using its RBD to bind with the cell surface receptor ACE2. The neutralizing antibody can bind with RBD of virus and prevent it from binding with ACE2, thus playing a protective role. Anti-SARS-CoV-2 Neutralizing Antibody ELISA Kit can help to check for the presence of protective neutralizing antibody.
Workflow
Dilute Samples/Controls with sample dilution buffer, and then mix with diluted HRP-RBD in supplied 96-well dilution plate
↓ 37°C incubation, 30 minutes
Transfer 1 OOμL of the samples/controls mixture to the corresponding wells of microplate that coated ACE2
↓ 37°C incubation, 20 minutes
Wash the well of the plate with diluted wash buffer for 4 times
↓
Add TMB substrate solution to each well of the plate
↓ 37 C incubation, 15 minutes
Add stop solution to each well, read immediately at 450 nm
Validation Data
Calculation:
| Inhibition= (1- | OD of Sample Mean OD of Negative control | ) X 100% |
Interpretation:
| Inhibition | ≥ 20% | Positive | Indicate the presence of SARS-CoV-2 neutralizing antibody |
| <20% | Negative | Indicate neutralizing antibody is absent or below the detection limit |
Order Information:
Cat. No | Specimen | Packing Size | Shelf Life | Storage |
C8909C | Serum/Plasma | 96 Tests/Kit | 10 Months | 2℃- 8℃ |
Key Advantages
• High-throughput
• Safe & Stable
• Time saving
Product Description
Many studies have proved that SARS-CoV-2 enters target cells by using its RBD to bind with the cell surface receptor ACE2. The neutralizing antibody can bind with RBD of virus and prevent it from binding with ACE2, thus playing a protective role. Anti-SARS-CoV-2 Neutralizing Antibody ELISA Kit can help to check for the presence of protective neutralizing antibody.
Workflow
Dilute Samples/Controls with sample dilution buffer, and then mix with diluted HRP-RBD in supplied 96-well dilution plate
↓ 37°C incubation, 30 minutes
Transfer 1 OOμL of the samples/controls mixture to the corresponding wells of microplate that coated ACE2
↓ 37°C incubation, 20 minutes
Wash the well of the plate with diluted wash buffer for 4 times
↓
Add TMB substrate solution to each well of the plate
↓ 37 C incubation, 15 minutes
Add stop solution to each well, read immediately at 450 nm
Validation Data
Calculation:
| Inhibition= (1- | OD of Sample Mean OD of Negative control | ) X 100% |
Interpretation:
| Inhibition | ≥ 20% | Positive | Indicate the presence of SARS-CoV-2 neutralizing antibody |
| <20% | Negative | Indicate neutralizing antibody is absent or below the detection limit |
Order Information:
Cat. No | Specimen | Packing Size | Shelf Life | Storage |
C8909C | Serum/Plasma | 96 Tests/Kit | 10 Months | 2℃- 8℃ |
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