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Anti-SARS-CoV-2 Neutralizing Antibody ELISA Kit

Many studies have proved that SARS-CoV-2 enters target cells by using its RBD to bind with the cell surface receptor ACE2. The neutralizing antibody can bind with RBD of virus and prevent it from binding with ACE2...
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Key Advantages

• High-throughput

• Safe & Stable

• Time saving


Product Description

Many studies have proved that SARS-CoV-2 enters target cells by using its RBD to bind with the cell surface receptor ACE2. The neutralizing antibody can bind with RBD of virus and prevent it from binding with ACE2, thus playing a protective role. Anti-SARS-CoV-2 Neutralizing Antibody ELISA Kit can help to check for the presence of protective neutralizing antibody.


Workflow

Dilute Samples/Controls with sample dilution buffer, and then mix with diluted HRP-RBD in supplied 96-well dilution plate

        ↓ 37°C incubation, 30 minutes

Transfer 1 OOμL of the samples/controls mixture to the corresponding wells of microplate that coated ACE2

        ↓ 37°C incubation, 20 minutes

Wash the well of the plate with diluted wash buffer for 4 times

         

Add TMB substrate solution to each well of the plate

        ↓ 37 C incubation, 15 minutes

Add stop solution to each well, read immediately at 450 nm


Validation Data

Calculation:


Inhibition= (1- OD of Sample
Mean OD of Negative control
) X 100%


Interpretation:

Inhibition ≥ 20% Positive

Indicate the presence of SARS-CoV-2 neutralizing antibody

<20% Negative

Indicate neutralizing antibody is absent or below the detection limit


Order Information

Cat. No

Specimen

Packing Size

Shelf Life

Storage

C8909C

Serum/Plasma

96 Tests/Kit

10 Months

2℃- 8℃


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