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2019-Novel Coronavirus (2019-nCoV) Triplex RT-qPCR Detection Kit

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This product provides triplex-detections in a single tube, including two independent genes of 2019-nCoV and an internal control which targets the human RNAse P (RNP) gene to assess specimen quality. Specifc primers and probes were designed for the detection of conserved region of 2019-nCoV’s ORF1ab gene and N gene, respectively, avoiding nonspecifc interference of SARS2003 and BatSARS-like virus strains. Internal control (RNAse P gene) provides a nucleic acid extraction procedural control and a secondary negative control. Positive control (2019-nCoV-pseudoviruse) provides a nucleic acid extraction and a reverse transcription control to validate the entire procedure and reagent integrity.

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